Based on the database of longan transcriptome sequences,the DlAG06 from longan embryogen⁃ic callus was isolated by using RACE and PCR. The results showed that the cloned full—length cDNA se⁃quence of DlAGO6 was 3 168 bp in length( Genbank:KF819529) ,containing a 2 700 bp open reading frame ( ORF) encoding 900 amino acids. Bioinformatics predicted that the deduced DlAGO6 protein with two con⁃served PAZ and PIWI domains had the typical characteristics of the AGO family,which had a high identity with the known Arabidopsis thaliana AGO6 protein. The qPCR analysis indicated that the expression level of DlAGO6 gene was the lowest at the stage of torpedo embryo and reached the maximum at the stage of the fria⁃ble⁃embryogenic callus,which suggested that DlAGO6 may be associated with exuberant cell division.%依据龙眼胚性愈伤组织转录组数据库Unigene序列，利用RT－PCR结合RACE技术，以龙眼胚性愈伤组织cDNA为模板，获得 DlAGO6基因的 cDNA 全长序列，共3168 bp （登录号为 KF819529），完整开放阅读框2700 bp，编码900个氨基酸。生物信息学分析表明：DlAGO6的cDNA所编码的氨基酸序列含有2个高度保守的PAZ和PIWI结构域，具有典型的AGO类蛋白的结构特征；与拟南芥AGO6蛋白序列有较高的同源性。龙眼体胚发生过程中DlAGO6表达量的qPCR分析表明： DlAGO6在龙眼松散型胚性愈伤组织时期的表达量最高，在鱼雷形胚时期表达量最低，推测DLAGO6在龙眼松散型胚性愈伤组织阶段的高表达量，可能与细胞的旺盛分裂有关。
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