为明确水稻黑条矮缩病毒(RBSDV)和南方水稻黑条矮缩病毒(SRBSDV)这2种病毒在江西省的分布,首先从永修县、南昌县和大余县等8县市采集水稻疑似矮缩病株,用2种病毒一步检测法对这2种病毒进行RT-PCR检测.结果表明:永修县所有样品均检出RBSDV,检出率为100％；南昌县同时检出RBSDV和SRBSDV,检出率分别为90％和20％,其中南昌县的1个样品同时检出2种病毒,存在复合侵染；大余县、莲花县、崇义县、婺源县、万安县和井冈山市等6县的所有样品均检出SRBSDV,检出率100％.然后,对这2种病毒在江西省的分布特点进行分析.统计分析结果表明:江西省西南部和东北部只有SRBSDV分布,江西省北部只有RBSDV分布,而处于以上3个病毒重发区之间的南昌县同时有RBSDV和SRBSDV分布.同时基于S9序列和S10序列建立这2种病毒及其近缘种的系统发育树,分析其亲缘关系.%To verify the distribution of two rice viruses, i. e. rice black-streaked dwarf virus and southern rice black-streaked dwarf virus, in Jiangxi, the suspected dwarf rice plants were gathered from 8 counties, i. e. Yongxiu, Nanchang, Dayu and so on, and were detected using the one-step detection method of 2 viruses. The results revealed that in all of the samples from Yongxiu County rice black-streaked dwarf virus was detected with a detection ratio of 100% , both rice black-streaked dwarf virus and southern rice black-streaked dwarf virus were detected in Nanchang County with the detection ratios of 90% and 20% , of which 2 viruses detected in the same sample, and all of the samples from Dayu County, Lianhua County, Chongyi County, Wuyuan County, Wan ' an County and Jinggangshan County were identified as southern rice black-streaked dwarf virus with a detection ratio of 100% . Furthermore, the regional distribution of these 2 viruses in Jiangxi was analyzed and the statistical analysis result revealed that there only existed southern rice black - streaked dwarf virus in the southwest and northeast of Jiangxi, there only existed rice black-streaked dwarf virus in the north of Jiangxi and there existed the two viruses in Nanchang at the junction of the above 3 disease regions. At the same time, phylogenetic trees among these two virus and their relatives were constructed based on the S9 and S10 sequences and their phylogenetic relationship was analyzed.
展开▼
