从大田油菜植株内获得117个细菌分离物,其中6个菌株对油菜菌核病菌(Sclerotinia sclerotiorum)表现不同程度的拮抗作用.经平板对峙培养和菌核萌发抑制率双重筛选出菌株JH-10,对油菜菌核病菌(S.sclerotiorum)具有较强的抑制作用,导致菌丝生长缓慢、弯曲、局部断裂,并延迟菌核形成的时间.菌株JH-10发酵液对油菜菌核病的离体叶片接种防效为73.1％,盆栽防效为64.7％.经几丁质平板检测,菌株JH-10发酵液中含有丰富的几丁质酶.本研究结果表明,内生细菌JH-10对油菜菌核病的生物防治具有较大的潜力,为几丁质酶基因的克隆提供重要材料.%One hundred and seventeen bacteria isolates were isolated from rapeseed plants grown in the fields, six of which were found having suppressing effect on Sclerotinia sclerotiorum mycelium growth in some degree. Strain JH-10, selected via double screening of plate stand - opposite culture and sclerotium germination rate, had an efficient suppressing effect on S. sclerotiorum, resulting in the fact that the mycelium grew slowly, bent or partly fractured, and the sclerotium production was delayed. Treated with fermentation broth of strain JH-10, the disease control efficacy was 73. 1% on rapeseed excised leaves, and 64.1% on pot trials. Fermentation broth of strain JH-10 produced rich chitinases by chitin plate testing. The results showed that en-dophytic bacteria JH-10 had great potentiality in biological control of S. sclerotiorum, and provided a substantial material for the cloning of chitinases.
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