以意大利青霉做指示菌,以抑菌圈直径为活性追踪指标,在单因素实验的基础上通过二次回归正交旋转组合设计进行凤仙透骨草中抑菌活性物质的提取工艺参数优化.结果表明:最佳提取工艺条件为φ(乙醇)=96.8%,溶剂倍数10倍,提取温度50 ℃,提取时间3 h,在此优化条件下抑菌圈直径为36.95 mm.各因素对凤仙透骨草提取物抑菌效果的影响由大到小依次为:乙醇浓度,溶剂倍数,提取时间,提取温度.%The diameter of inhibition zone against Penicillium italicum was used as activity tracking indicators to investigate the technology for extracting antimicrobial components from stems of Impatiens balsamina.The optimum parameters of the extracting technology were obtained by quadric regression orthogonal rotational experiments. The results showed that: the best extraction conditions were: the ethanol concentration was 96. 8℃,solvent times were 10, extraction temperature was 50 ℃, extraction time was 3 h. The diameter of inhibition zone against Impatiens balsamina was 36.95 mm when inhibited by the extracts extracted under the above mentioned conditions. The order of the main factors influencing the extracting effect from more important to less is as follows: ethanol concentration, solvent times, extraction time and extraction temperature.
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