The albumin and globulin of 2005 -00 and Qinghai 13, two varieties of Qinghai Faba bean were studied by improved SDS - PAGE. At the same time by using micro - Kjeldahl Method the appropriate protein content were measured. The result showed that the content of globulin was twice that of albumin.Therefore globulin was the main component of the total protein content. Almost all the albumin and globulin were extracted after extraction for five times with extract solution Ⅰ which included NaCl. But only the globulin were obtained with extract solution Ⅰ for one time after with extraction for four times extract solution Ⅱ which didn't include NaC1, and the globulin had the same mobility as the albumin. The result indicated that albumin was also extracted while extracting globulin, but globulin was not extracted while extracting albumin.%利用改进的SDS-聚丙烯酰胺凝胶电泳,对2种青海蚕豆品种2005-00和青海13号的清蛋白、球蛋白进行研究,并采用微量凯氏定氮法测得相应的蛋白质含量.结果表明:球蛋白含量比清蛋白含量高,是总蛋白含量的主要成分.用含有NaCl的球蛋白提取液Ι提取5次蛋白质后,清蛋白和球蛋白被同时提取出来;而用不含NaCl的清蛋白提取液Π提取4次蛋白质后,再用提取液Ι提取,可得到球蛋白组分,而且和清蛋白一样也有相同迁移位置的谱带存在.表明对球蛋白进行提取操作时,总有清蛋白相伴随;而对清蛋白进行提取分析时一般不会有球蛋白的存在.
展开▼
