换锦花是我国南方的优良球根花卉和重要的药用植物.采用组织培养技术以换锦花种胚和叶片为外植体,对其进行研究,结果显示:换锦花种胚的诱导率在各培养基上均较高,平均为75.8％,适宜的愈伤组织诱导培养基为MS+6- BA 2 mg/L+ 2,4 -D2 mg/L+蔗糖60 g/L,适宜的丛芽诱导培养基为MS+6- BA 2 mg/L+2,4-D 1 mg/L+蔗糖30 g/L；换锦花成熟叶段愈伤组织的平均诱导率为13.5％,而幼嫩叶段的平均诱导率为65.8％,两者差异极显著,适宜于幼嫩叶段诱导的培养基为MS+6- BA 20 mg/L+ NAA 1 mg/L+蔗糖30g/L,各生长调节剂配比的试验结果显示,NAA较高时利于根的诱导,而6- BA较高时利于芽的诱导.%Lycoris sprengeri Comes ex baker is one of the most beautiful bulbs but hasn' t been used in landscaping widely owing to lack of rapid propagation technique. The leaf blades and embryo of L. Sprengeri were cultured in vitro on MS mediums supplemented with different concentrations of plant growth regulator. The results showed that high induction rates, 75. 8% on average, were obtained in embryo culture. The most ideal callus induction medium for embryo culture was MS + 6 - BA 2 mg/L + 2,4 - D 2 mg/L + Sucrose 60 g/ L, while the most ideal clustered shoots induction medium was MS + 6 - BA 2 mg/L + 2,4 - D 1 mg/L + Su crose 30 g/L. The results of leaf tissue culture showed that the induction rate of young leaves was 65. 8% on average which was higher than that of mature leaves( 13. 5% on average). The most ideal induction medium for young leaves tissue culture was MS + 6 - BA 20 mg/L + NAA 1 mg/L + Sucrose 30 g/L. The results of growth regulator contrast test showed that higher NAA concentration was good for induction of roots, while higher 6 - BA concentration was good for induction of shoots.
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