In the experiment, 200 chickens were randomly divided into 4 groups (50 chichens each group) : the control group, the NDV vaccine group, the NDV vaccine + recombinant IL-18 group, the NDV vaccine + vector control group. All the groups were immunized by its vaccine respectively on the eighth day. Then respectively at the third day, 7th day, 14th day, 21st day, 28th day and 35th day after immunization, from each group 4 chickens were taken randomly to detect their antibody level and IFN - γ concentration in seurm, and T cell activity ratio. Twenty chickens of each group were used for challenge and protection test on the eighteenth day after immunization. The results indicated that rIL-18 could improve the protective rate of ND vaccine. It had obvious immunoenhancement effect on Newcastle disease vaccine. It could not only enhance the cell - mediated immune response obviously, but also enhance the humoral response.%将200只1日龄鸡随机分为4组,每组50只鸡,分别为空白对照组,新城疫疫苗组,新城疫疫苗+重组IL-18组,新城疫疫苗+空载体对照组.所有免疫组鸡均于8日龄免疫接种相应疫苗,并分别于免疫后的第3,7,14,21,28天和第35天对各组鸡随即抽取4只,对其血清的抗体效价、外周血的T淋巴细胞转化水平和γ干扰素含量进行测定.免疫18 d后每组鸡随机取20只进行动物攻毒保护试验.结果表明重组鸡IL-18蛋白不仅能够显著增强新城疫疫苗所诱导的细胞免疫和体液免疫反应,而且还可以明显提高疫苗的保护率.
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