用正交试验设计法研究了6-BA,NAA和2,4-D 3种植物生长调节剂对根茎秋海棠‘Helen Lewis’叶片诱导不定芽的影响,建立了根茎秋海棠叶片再生体系.结果表明,诱导根茎秋海棠不定芽发生的最佳配方为:MS +2 mg·L-1 6-BA +0.5 mg·L-1 NAA +0.5 mg·L-12,4-D,36 d时诱导率为86.7％,并有淡绿色愈伤组织形成.附加0.5 mg· L- 6-BA+0.2 mg· L-1 NAA的MS培养基有利于不定芽的分化和增殖.在1/2 MS+0.5 mg· L- IBA的培养基中生根效果最好,高于4 cm的再生植株移栽成活率达90％以上.%Effects of 6-BA, NAA and 2,4-D on adventitious buds induction from leaf explants of Begonia Helen Lewis were studied by L, ( 34) orthogonal design. In the present work we established an in vitro regenerative protocol for the large-scale propagation. The experimental results showed that the best medium for adventitious buds induction was MS with 2 mg'L"1 6-BA,0.5 mg-L~' NAA, 0.5 mg'L"' 2,4-D, and young leaves used as explants showed 86. 7% induction rate of adventitious buds for about 36 d, with light green calli formed. The MS media supplemented with 0. 5 mg-L'1 6-BA and 0. 2 mg-L~l NAA was beneficial to differentiation and proliferation of adventitious buds and calli, while the best rooting effect was in 1/2 MS medium with 0. 5 mg-L IBA. Plantlets longer than 4 cm were successfully transplanted into soil medium showing a survival rate of 90%.
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