To construct mutant library of physical mutation in broomcorn millet, applied five doses of 12C6+ ion beam at 50, 100, 150, 200, and 250 Gy to irradiate seed of Longmi 7 and Jinshu 9, and constructed two populations (M5) consisting of 52 and 79 lines respectively with mixed pedigree method and system cluster. The field text showed that the emergence rate of M1 decreased significantly with increasing 12C6+ dose, half lethal dose for M1 from Longmi 7 and Jinshu 9 were 150 Gy and 100 Gy. M4 at 100, 150 Gy produce a most abundant mutants. M5 and M6 Phenotypes were more stable than M4, and plant height, yield traits, plant color and seed color showed significant difference. Nine M6 lines from Longmi 7 and 11 M6 lines from Jinshu 9 were detected with six pairs of SSR primer, compared with the parents, loci variant genotype number were 1–2 and 1–4, respectively, showing abundant genetic diversity in mutant populations.%为构建物理诱变糜子突变体库,采用50、100、150、200和250 Gy剂量碳离子束(12C6+)辐照陇糜7号和晋黍9号种子,结合混合系谱法和系统聚类分别构建2个含52和79个株系的M5寡表型突变群体.田间试验结果显示,诱变M1出苗率随剂量增大明显降低,陇糜7号M1半致死剂量为150 Gy,晋黍9号M1半致死剂量为100 Gy,且100、150 Gy诱变下M4变异最多.不同诱变剂量下M5、M6表型稳定且株高、产量性状、成株色及粒色均表现出明显遗传差异.从2个M6群体中分别选择9和11个代表性株系,利用多态性SSR引物进行分子验证,与亲本相比,6对SSR引物在陇糜7号的9个株系中位点变异基因型数为1～2,在晋黍9号的11个株系中位点变异基因型数为1～4,突变群体存在丰富的遗传多样性.
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