In plants, RNase can degrade RNA to release Pi in senescent organs, which can be reused by young organs. The expression of many S-like RNase genes were up-regulated by phosphate starvation. Using the ESTs, three S-like RNase gene cDNAs were isolated from common wheat by RT-PCR. The predicated amino acid sequences of the three sequences were found having high similarities with those S-RNases and S-like RNases in other plant species. The expression of WRN1 was down-regulated by phosphate starvation and leaf senescence, while WRN2 and WRN3 were up-regulated by phosphate starvation.%核糖核酸酶(RNases)可以将衰老的植物组织中的核糖核酸降解释放出磷元素,使它能够运送到幼嫩部位被重新利用.许多核糖核酸酶基因的表达受磷饥饿的正调控.利用已有的EST序列,从普通小麦"小偃54"中分离了3个核糖核酸酶基因的cDNA序列.这3个基因预测的氨基酸序列与S-核糖核酸酶和S-like核糖核酸酶(类核糖核酸酶)的氨基酸序列有较高的同源性.WRN1的表达受磷饥饿和衰老的负调控,而WRN2和WRN3受磷饥饿的正调控.
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