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Ca2+对低温下水稻幼苗膜的保护作用

         

摘要

In cold temperature condition (4℃ 36h), if the rice seedling growin the solution of leak Ca2+, the functions of cell membrane and the ultrastruture of membranes of chloroplast and mitochondria were damaged gravely. When lower consistence Ca2+ was added in the solution, it were observed that electrolyte leakage and malondialdehyde (MDA) contents decreae and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) increased and the membrane structure of chloroplast and mitochondria were better. Chlorpromazine (CPZ), a specific inhibitor of CaM, would inhibit Ca2+ function to decreasing electrolyte leakage and MAD content and increasing SOD, CAT and POD activities of chilling seedling. The result suggests that there is a relationship between Ca2+ funtion and CaM.%缺Ca2+的水稻幼苗在低温胁迫下(4℃36h),细胞膜功能及超微结构破坏严重。在培养液中加入适当浓度的Ca2+(0.5~1.0mmol/L)可降低冷胁迫下稻苗的电解质渗漏率和MDA含量,提高SOD、CAT、POD活性,保护叶绿体和线粒体超微结构免遭破坏。CaM特异性抑制剂CPZ能部分抑制Ca2+降低冷害稻苗的电解质渗漏率及MDA含量和提高SOD、POD活性的作用,暗示Ca2+对水稻幼苗膜的保护作用与CaM有一定关系。

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