用RAPD技术对细胞质分别来源于粘果山羊草（Ae. kotschyi）、偏凸山羊草（Ae. ventricosa）、提莫菲维小麦（T. timopheevii）的三种普通小麦雄性不育系--K型、 V型、 T型及相应的保持系、恢复系以及杂种F1代的线粒体DNA（mtDNA）进行了比较分析。结果如下：（1）发现在mtDNA组织结构上K型、 V型、 T型不育系之间以及与保持系之间均呈现差异，这从DNA水平上提供了三类不育系胞质来源不同的证据；（2）利用23个引物的扩增结果进行了聚类分析，绘制出了T型、 K型、 V型三种不育系、保持系和K、 V型共同的恢复系及杂种F1代树式遗传关系图；（3）对两个特异的多态性DNA片段进行了回收，克隆、并制备探针， Southern杂交结果显示不育系与保持系间存在多态性，有两个片段可能与育性有一定的联系。%Mitochondrial DNAs(mtDNAs) from three types of cytoplasmic male sterility lines (CMS-K, -V and -T) in wheat, of which the cytoplasm derived from Ae. kotschyi, Ae. ventricosa and T. timopheevii, respectively, were compared among CMSs and their maintainer, restorer and hybrids of CMS-K and -V. The results indicated that: (1) the mtDNAs from CMS-K, -V and -T were different from each other and from their maintainer, this provided some evidence at molecular level for three kinds of CMS lines with different cytoplasms; (2) the genetic dendrogram was made and the relative genetic distance among 7 different wheat materials was calculated by cluster analysis with amplification products from 23 primers; (3) two specific fragments of mtDNA were then cloned into plasmid vector and converted into RFLP probes.Southern hybridization patterns revealed polymorphism between sterile line and maintainer line .
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