首页> 中文期刊> 《生物化学与生物物理学报:英文版》 >BAF Complex Is Closely Related to and Interacts with NF1/CTF and RNA Polymerase Ⅱ in Gene Transcriptional Activation

BAF Complex Is Closely Related to and Interacts with NF1/CTF and RNA Polymerase Ⅱ in Gene Transcriptional Activation

         

摘要

Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg 1 is the core subunit of Brg-associated factor complexes. In the present study, we investigated the spatial relationship between Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase Ⅱ (RNAP Ⅱ) upon gene transcriptional activation in vivo by employing immuno-gold labeling. The data showed that Brg1 was closely co-localized with NF1/CTF and RNAP Ⅱ in HeLa cells. Moreover, the co-immunoprecipitation assay further revealed that Brg1 can be isolated together with NF1/CTF and RNAP Ⅱ in the ConA-stimulated, but not the resting,T lymphocyte. The combined results suggested that BAF complexes can interact with NF1/CTF and RNAP Ⅱ, and this interaction is closely dependent on the activation of gene transcription.

著录项

  • 来源
    《生物化学与生物物理学报:英文版》 |2005年第7期|440-446|共7页
  • 作者单位

    Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;

    College of Agronomy, Jilin Agricultural University, Changchun 130118, China;

    Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;

    Department of Biology, Changchun Normal College, Changchun 130032, China;

    Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;

    Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;

    Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 生物化学;
  • 关键词

    BAF complex; NF1/CTF; RNAP Ⅱ; gene transcription;

    机译:BAF复合物;NF1 / CTF;RNAPⅡ;基因转录;
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