The dynamic interaction process of biotin-avidin was investigated by biotin covalently modified microcantilever sensor in real-time in a flowing cell. The specific interaction was confirmed by the evidence that the avidin layer can be mechanically removed with atomic force microscopy nanolithography. The deflection of the modified cantilever increased with concentration of avidin. Microcantilever biosensor provides a good method to explore novel label-free technology and monitor various interactions between biomolecules.%将生物素共价键合到微悬臂的硅表面,利用微悬臂传感技术在流动注样条件下研究了生物素-亲和素的动态作用过程,同时利用原子力显微镜(AFM)力刻蚀技术验证了生物素化硅表面结合亲和素的有效性.结果显示亲和素与生物素作用产生较大的表面应力驱动微悬臂偏转,悬臂偏转的程度和速率与亲和素的浓度相关,亲和素浓度越大,微悬臂偏转程度也越大,同时反应速率也更快.这项工作为开发新型的无标记检测技术,研究生物分子之间相互作用提供了有益的探索.
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