The fingerprint chromatograms of the extracts from Compound Indigowoad Root Granule was established by HPLC-UV.Nineteen common peaks were found in fingerprint chromatograms of the extracts from Compound Indigowoad Root Granule and good similarities with correlation coefficients over 93%were found between the fingerprint chromatograms of the extracts from different sources.The main common peaks were identificated by HPLC-MSn.Pharmacodynamic studies have shown that the extract from Compound Indigowoad Root Granule had anti-viral activity.This method with desirable accuracy, stability and repeatability can be used for the quality evaluation of Compound Indigowoad Root Granule.%建立了复方板蓝根颗粒提取物高效液相色谱(HPLC-uv)指纹图谱分析方法.确定了10批不同来源复方板蓝根颗粒提取物的19个共有峰.各提取物的HPLC-UV指纹图谱与对照指纹图谱比较,相似度均在93%以上.利用液相色谱与质谱联用(LC-ESI-MSn技术对主要共有峰的结构进行了鉴定.药效学研究表明,复方板蓝根颗粒提取物有抗病毒活性.该指纹图谱精密度、稳定性和重现性良好,可作为复方板蓝根颗粒的质量评价方法.
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