通过一步法制备了稳定、分散的聚多巴胺包被的银纳米粒子（AgNPs）.基于Hg2＋与聚多巴胺的吸附作用使AgNPs发生聚集,导致体系吸收信号发生变化,同时溶液颜色也发生相应改变,建立了Hg2＋的紫外-可见分光光度法及色度法.线性范围为0.5～5.0μmol/L,检出限为50 nmol/L.表征了紫外-可见光谱、红外光谱及扫描电镜显微成像（SEM）,优化了实验条件,并探讨了反应机理.该方法用于实际样品中Hg2＋的测定,RSD≤4.4%.%In this work,the colorimetric detection assay of mercury has been proposed based on silver nanoparticles（AgNPs） aggregation.In basic medium with 1.75 mmol/L NaOH,the stable and monodisperse silver nanoparticles,which coated by polydopamine,was prepared by a one-pot reduction process using dopamine as a reducing agent.The polydopamine adsorption with Hg2＋ can induce the aggregation of silver nanoparticles resulting in the decrease of absorption signal of AgNPs at 405 nm and the color change of the solutions.It was found that the decreased absorption signal intensity was proportional to the mercury content in a range of 0.5～5.0 μmol/L.According to this,the ultraviolet-visible absorption spectrum method and colorimetric method for determination of Hg2＋ was developed.The limit of detection is 50 nmol/L.In this work,UV-vis absorption spectrum,scanning electron microscope（SEM） and infrared（IR） spectrum were investigated.The mechanism of this reaction was discussed.The IR spectrum of special functional groups approved that polydopamine was formed on the surface of AgNPs.The UV-Vis absorption peak at 405 nm and SEM photos indicated that the stable and monodisperse AgNPs was prepared and the aggregation of AgNPs was occurred after the addition of Hg2＋.The tolerance of coexisting foreign substances in system was also studied and the experimental results indicated that the interferences of other common metal ions were small.The experimental condition optimization results show that when the NaOH concentration is 1.75 mmol/L and dopamine concentration is 40 μmol/L,the system has a good response for Hg2＋.The proposed method was successfully used to detection of Hg2＋ in tap water with a recovery range of 93.0% to 105.5% and RSD≤4.4%.This method show some advantages including low-cost,rapidity,simplicity and selectivity.Moreover,it can achieve a visual sensing of Hg2＋.
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