Sustained release carrier for the bone morphogenetic protein-2 （BMP-2） is currently a hot subject of research on the bone tissue engineering. In this work, two kinds of heparinized silk fibroin scaffolds were prepared. The binding ability to BMP-2, in vitro sustained release property and the alkaline phosphatase （ALP） activity of the scaffolds were investigated. Results showed that the heparinized silk fibroin scaffolds could bind BMP-2 well and sustained release the BMP-2. The scaffolds with BMP-2 could improve the MG-63 cell growth and cell differentiation with high ALP activity. So the heparinized silk fibroin scaffolds could be an ideal sustained release carrier for BMP-2.%骨形态发生蛋白-2（BMP-2）的缓释载体一直是骨组织工程中的研究热点.本研究通过化学改性制备了两种肝素化丝素支架,并浸渍吸附BMP-2,研究了BMP-2在不同丝素支架样品上的吸附能力、体外释放性能及其对人骨肉瘤细胞MG-63碱性磷酸酶活性（ALP）的影响.结果表明,肝素化丝素支架对BMP-2具有较强的吸附能力,并能保持其体外缓慢释放性能;MG-63细胞在肝素化支架上生长状态良好,并具有显著的增殖能力,负载BMP-2后的肝素化支架能显著促进MG-63细胞的分化.因此,肝素化丝素支架是一种较理想的BMP-2缓释载体.
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