Objective:To investigate the effect of Paeonol on the proliferation and COX -2 transcription of SiHa cells . Methods:Human cervical squamous cell carcinoma SiHa cell line was used as a model in vitro .The proliferation of SiHa cells treated by Paeonol was detected by MTT assays .Plasmids pCOX2-Luc and pAP1-Luc were transfected into SiHa cells and the dual luciferase reporter gene assay system was used to detect the transcription level of COX -2 and the ac-tivity of AP-1 .Results:The results of MTT assays showed that Paeonol significantly inhibited the proliferation of SiHa cells and the effect was associated positively with the dose and treatment time of paeonol .Luciferase assay results showed that Paeonol apparently reduced the transcription level of SiHa COX -2 cells ( P<0 .01 ) , and obviously inhibited the activity of AP-1(P<0.01).Conclusion:Paeonol may inhibit COX-2 transcription by depressing the activity of tran-scription factor AP-1,thereby suppress the proliferation of SiHa cells .%目的：探讨丹皮酚对SiHa细胞增殖及COX－2转录的影响。方法：以人宫颈鳞癌SiHa细胞系作为体外模型，MTT法检测丹皮酚对SiHa细胞增殖的影响。用COX－2启动子质粒和AP－1结合位点质粒转染SiHa细胞，采用双荧光素酶报告基因检测系统检测COX－2的转录水平和AP－1的活性。结果：MTT实验结果显示丹皮酚显著抑制SiHa细胞的增殖（ P＜0．01），且与丹皮酚剂量及干预时间呈正相关。荧光素酶检测结果显示丹皮酚显著降低SiHa细胞COX－2的转录水平（ P＜0．01），同时显著抑制AP－1的活性。结论：丹皮酚可通过抑制转录因子AP－1的活性，进而抑制COX－2的转录，从而抑制SiHa细胞的增殖。
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