观察三痹颗粒对Ⅱ型胶原诱发类风湿关节炎（CIA）模型大鼠病变关节细胞趋化因子受体的影响，探讨其抗炎作用机制。方法：将 CIA 模型大鼠随机分为模型组、三痹颗粒高剂量组、三痹颗粒中剂量组、三痹颗粒低剂量组、另取空白组。三痹颗粒高、中、低剂量组于初次免疫第15天分别给予三痹颗粒蒸馏水溶液灌胃，模型组和空白对照组灌服同体积的生理盐水，连续灌服20天，观察大鼠足踝关节肿胀度以及关节炎指数的变化，RT -PCR 法测定大鼠外周血趋化因子受体3（CXCR3）、趋化因子受体4（CXCR4）mRNA 含量。结果：第21天，与空白组比较，模型组及三痹颗粒各组足容积明显增大，差异有统计学意义（P ＜0．05）；第35天时，与模型组比较，三痹颗粒高、中剂量组足容积的上升程度减慢，进一步减轻关节炎症反应程度，差异有统计学意义（P ＜0．05）。与模型组比较，中药各剂量组大鼠关节炎评分指数明显降低，差异有统计学意义（ P ＜0．05）。与模型组比较，三痹颗粒各组大鼠外周血CXCR3mRNA、CXCR4mRNA 的表达明显降低，差异有统计学意义（P ＜0．05）。结论：三痹颗粒能够抑制CIA 模型大鼠外周血 CXCR3、CXCR4的表达，从而减慢 CIA 模型大鼠足容积的上升程度，降低关节炎指数，抑制炎症反应。%Objective:To observe the effect of Sanbi granule on chemokine receptor of diseased joints in CIA model rats , and to discuss the anti -inflammatory mechanism .Methods:Divide CIA model rats randomly into a model group ,a high dose group ,a mid-dose group ,a low-dose group and a blank group .In high dose group ,mid-dose group and low -dose group model rats were given different doses of Sanbi granule distilled solution in primary immune on the 15th day. In model group and blank group model rats were given the same volume normal saline .All model rats were gavaged for 20 days.Observe model rats ’ swelling ankles and the arthritis index .Measure the contents of CXCR 3 and CXCR4 in model rats with RT -PCR method.Results:Compared with blank group on the 2lst day, in model group and Sanbi granule groups the model rats ’ feet were all increased in volume .The difference had a statistical significance ( P<0 .05 ) .Com-pared with model group on the 35th day,the rising degree of model rats ’ feet volume in Sanbi granule groups slowed down .And in these groups the degree of joint inflammation was reduced .The difference had a statistical significance ( P<0.05 ) .Compared with model group ,the arthritis index of Sanbi granule groups was decreased sharply .The differ-ence had a statistical significance (P <0.05).Compared with model group,the expressions of CXCR3mRNA,CX-CR4mRNA of model rats ’ peripheral blood in Sanbi granule groups were decreased obviously .The difference had a sta-tistical significance(P<0.05).Conclusion:Sanbi granule can restrain the expressions of CXCR 3,CXCR4 of peripheral blood in CIA model rats ,make the rising degree of model rats ’ feet volume to slow down ,decrease arthritis index and re-strain inflammatory reaction .
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