目的 探讨H9N2亚型猪流感病毒诱导小鼠急性肺损伤过程中炎症因子的变化和作用.方法 通过滴鼻的方法将H9N2亚型猪流感病毒感染BALB/c小鼠,于感染后2、4、6、10、14 d取小鼠肺组织匀浆,分别测定肺组织匀浆中TNF-α、IL-1β、IL-6和IL-10的浓度.结果 实验组肺组织匀浆中TNF-α、IL-1β、IL-6和IL-10在不同时间点浓度均显著高于正常对照组(P＜0.05),TNF-α和IL-1β于14 d后趋于正常,而IL-6和IL-10增高持续至14d后.结论 H9N2猪流感病毒诱导小鼠急性肺损伤过程中炎症因子发挥重大作用,TNF-α和IL-1β起致炎作用,IL-6可能和IL-10一样,发挥抗炎作用.%Objective To explore the changes and roles of inflammatory factors in acute lung injury induced by swine influenza virus H9N2 in mice. Methods Eighty 6-8 week old female Balb/c mice were divided into experimental and control groups (n =40). The mice of experimental group were infected with swine influenza virus (H9N2) by nose dropping. Lung tissue specimens of the mice were taken on day 2, 4, 6, 10 and 14 after infection, and the concentrations of TNF-α, IL-1β, IL-6 and IL-10 in the tissue homogenate were determined. Results Concentrations of TNF-a, IL-1β, IL-6 and IL-10 in the lung tissue homogenate of infected mice were significantly higher than that in the control group at different time points (P < 0. 05 ) . TNF-α and IL-1β were tended towards normal, but IL-6 and IL-10 were still at high levels after 14 days. Conclusions Inflammatory factors play a major role in acute lung injury induced by H9N2 subtype swine influenza virus in mice. TNF-a and IL-1β display a proinflammatory effect, while IL-10 is likely to play an anti-inflammatory role, similar to that of IL-6.
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机译：通过使用小鼠盲肠结扎和穿刺诱导的败血症模型/ Compararea efectelor anti-inflamatoare alesimvastatineişiRosuvastatineimăsurândniveleleserice ale IL-测量IL-1β，IL-6和TNF-α水平来比较辛伐他汀和瑞舒伐他汀的抗炎作用。 1β，IL-6 si TNF-αfolosind un model de sepsislaşobolanususprinlactaturăşipuncţiececală
