目的 观察海人酸(kainic acid,KA)所致癫痫(epilepsy,EP)小鼠海马Ste20蛋白激酵素(MST3)表达水平的变化,探讨MST3在癫痫发病过程中的可能作用.方法 选用成年雄性小鼠,并随机分成模型组和对照组.模型组小鼠侧脑室注射2 μL(100 ng/μL)KA,分别于术后3、8、24 h收集动物标本以进行检测.使用RT-PCR和Western Blot测定MST3mRNA含量和MST3蛋白动态表达变化,应用免疫组化观察MST3在海马的表达分布与特点.结果 与正常对照组相比,模型组海马组织内MST3mRNA的表达随时间持续升高,24 h达到高峰；MST3的蛋白表达也表现出同样的动态升高趋势；术后3 ～24 h的模型组海马免疫组化检测显示,模型组MST3主要以海马齿状回、门回区、CA3区域表达增加为主,并且这些区域表达逐渐递增.结论 随着时间的推移,MST3表达水平呈现逐渐增加趋势,可能与神经元损伤造成的凋亡之间有密切的关系,提示MST3可能在癫痫发病过程中起重要作用.%Objective To investigate the expression of mammalian STE20-like protein kinase 3 ( MST3) in the hippocampus of epileptic mice induced by kainic acid ( KA ) , and to explore the role of MST3 in pathogenesis of epilepsy. Methods Adult male mice were randomly divided into control and experimental groups. The experimental group was injected with 2 ΜL ( 100 ng/μL) KA in the lateral cerebral ventricle and detected at 3 ,8,24 hours after injection. The levels of mRNA and protein were detected by RT-PCR and Western blot, respectively. The dynamic changes of MST3 in the hippocampus were revealed by immunohistochemistry. Results Compared with the control group, both the levels of MST3 mRNA and protein in hippocampus of the experimental group were increased gradually over time and reached a peak at 24 hours after KA injection. The results of immunohistochemistry showed that the MST3 expression in the hippocampus of experimental group was increased, mainly in the dentate gyrus and CA3 area. Conclusions The MST3 expression in epileptic mice increases gradually during the disease progression, and may be related with apoptosis in neurons. MST3 may play an important role in epilepsy pathogenesis.
展开▼
