Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge

WU Yuehong; CAO Yi; WANG Chunsheng; WU Min; AHARON Oren; XU Xuewei

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Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge

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A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction(PCR) amplification of the 16 S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E(MBGE) and marine group I(MGI) belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilonproteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the Southwest Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III NifH were present. This is consistent with the phylogenetic analysis of the microbial 16 S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.

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《海洋学报：英文版》 |2014年第10期|94-104|共11页
作者
WU Yuehong; CAO Yi; WANG Chunsheng; WU Min; AHARON Oren; XU Xuewei;
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作者单位

Laboratory of Marine Ecosystem and Biogeochemistry;

Second Institute of Oceanography;

State Oceanic Administration;

State Key Laboratory of Microbial Resources;

Institute of Microbiology;

Chinese Academy of Sciences;

College of Life Sciences;

Zhejiang University;

Department of Plant and Environmental Sciences;

the Alexander Silberman Institute of Life Sciences;

The Hebrew University of Jerusalem;

Jerusalem 91904;

Israel;

The Hebrew University of Jerusalem;

The Edmond J. Safra Campus;

Givat Ram;

Jerusalem 91904;

Israel;

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原文格式 PDF
正文语种 chi
中图分类生物固氮、游离氮的同化作用;技术方法;
关键词
微生物群落结构; 热液喷口; 生物固氮; 洋中脊; 沉积物; 基因多样性; 印度; 西南;

Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge

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