Improved method for measuring the δ15N compound-specific amino acids: Application on mesopelagic fishes in the South China Sea

Fuqiang Wang; Ying Wu; Lin Zhang; Jie Jin; Zuozhi Chen; Jun Zhang; Wing-man Lee

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Improved method for measuring the δ15N compound-specific amino acids: Application on mesopelagic fishes in the South China Sea

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Compound-specific stable isotope analysis of individual amino acids(CSIA-AA)has been widely used in ecological and biogeochemical studies.It has been proven to be powerful in tracing the diet sources and trophic interactions.However,assessing the N sources of mesopelagic fishes has been inconclusive because the mesopelagic fishes’unique domain(water depth ranged from 0 to 1000 m)and unresolved nitrogen isotopes of various forms.This study proposes a new method for coupling instruments(ion chromatography and PreconIRMS)and chemical method of oxidation-reduction of amino acids,and also combinedδ15N of AAs withδ13C of fatty acids(FAs)to analyze the trophic interactions of mesopelagic fishes in the South China Sea(SCS).AAs were isolated by ion chromatography with high peak resolution and collected by an automated fraction collector.The chemical method then converted the AAs into N2 O with a robust oxidation yields and suitable molar ratio of NH2 OH to.Finally,theδ15N of AAs at 20 nmol were measured with a reasonable precision(<0.6‰).With this method,this study report the first batch high precisionδ15N of AAs andδ13C of FAs of mesopelagic fishes collected from SCS.Diaphus luetkeni,Chauliodus minimus and Bathygadus antrodes showed similarδ13C values of 20:4 n-6(~-28‰),while Argyropelecus affinis and Stomias had similar values(~-32‰).These results reflect that mesopelagic fishes had complex diet sources.An increase of 4‰inδ15N of glutamic acid(Glu)was found between piscivorous and planktivorous fishes,which might suggest a trophic discrimination factor of mesopelagic fishes in the SCS.This study usedδ13C of 20:4 n-6 to reveal the diet sources of mesopelagic fishes andδ15N of Glu to clarify trophic level between piscivorous and planktivorous fishes.Thus,this combinative method could therefore ultimately be applied in a variety of deep-sea ecosystem.

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来源
《海洋学报：英文版》 |2022年第1期|30-38|共9页
作者
Fuqiang Wang; Ying Wu; Lin Zhang; Jie Jin; Zuozhi Chen; Jun Zhang; Wing-man Lee;
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作者单位

State Key Laboratory of Estuarine and Coastal Research;

East China Normal University;

Shanghai 200241;

China;

Center for Blue Life;

Pilot National Laboratory for Marine Science and Technology(Qingdao);

Qingdao 266237;

China;

Department of Physical and Environmental Science;

Texas A&M University-Corpus Christi;

Texa 78412;

USA;

Key Field Scientific Experimental Station of South China Sea Fishery Resource and Environment;

Ministry of Agriculture and Rural Affairs;

Guangzhou 510300;

China;

South China Sea Fisheries Research Institute;

Chinese Academy of Fishery Sciences;

Guangzhou 510300;

China;

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原文格式 PDF
正文语种 chi
中图分类有机化学;
关键词
δ13C of fatty acids; δ15N of amino acids; ion chromatography; mesopelagic fishes; Precon-IRMS;

Improved method for measuring the δ15N compound-specific amino acids: Application on mesopelagic fishes in the South China Sea

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