The study established a simple,rapid and accurate reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for zika virus (ZIKV).Three sets of LAMP primers were designed with online tools.The visual RT-LAMP assay was established with the optimized primers selected by real-time turbidimeter and Hydroxy naphthol blue (HNB),which possessed high sensitivity and specificity.The detection limit of this method was 10 copies/reaction,which was 10-100 fold higher than the conventional PCR and quantitative real-time PCR.The method was high specificity without any cross-reaction with other arbovirus.This method provided an experimental tool for the arbovirus monitoring and clinical diagnosis.%本研究建立了一种简单、快速、准确的寨卡病毒可视化逆转录环介导等温扩增(RT-LAMP)检测方法.在线设计3套LAMP引物,利用实时浊度仪筛选最佳引物,加入羟基萘酚蓝,评估可视化RT-LAMP检测方法的灵敏度和特异性.建立的可视化RT-LAMP方法可检测的最低浓度为101 copies/μL,高于普通PCR和荧光定量PCR 1~2个数量级;同时,该方法不与其他虫媒病毒产生交叉反应.该方法可为虫媒监测和临床诊断提供实验依据.
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机译：Development of an One-step Reverse Transcription Loop-mediated lsothermal Amplification Method for Rapid Detection of Bovine Viral Diarrhea Virus牛病毒性腹泻病毒一步 RT-LAMP快速检测方法的建立
