微线体蛋白2（Microneme protein 2， Mic 2）是艾美耳球虫入侵宿主细胞时分泌的主要功能蛋白之一。本研究中，以抗柔嫩艾美耳球虫微线体蛋白2（ EtMic2）单克隆抗体为检测抗体，利用间接免疫荧光实验（ IFA）检测了Mic 2在柔嫩艾美耳球虫、和缓艾美耳球虫、斯氏艾美耳球虫和无残艾美耳球虫等不同种艾美耳属球虫的空间分布。结果显示， Mic 2在不同种艾美耳属球虫中均定位于子孢子的顶部（微线体部位），但表达强度存在差异。这提示Mic 2可作为研究艾美耳属球虫相关蛋白空间分布的“参照物”。%Microneme protein 2 ( Mic 2) is the structural protein of microneme of Eimeria spp.The secretion of Mic 2 is very important during the process of invading the host cells.In this study, we used a monoclonal antibody specific to E. tenella Mic 2 ( EtMic2) to detect the Mic 2 location in several Eimeria spp., i.e., E.tenella, E.mitis, E.stiedai and E. irresidua, by indirect immunofluorescence assay (IFA).The Mic 2 was located in the anterior region of the sporozoites among all Eimeria spp., which indicated that Mic 2 is highly conserved among Eimeria spp.and could be used as a marker protein when studying the location of Eimeria spp.proteins.
展开▼
