Aim To study the effects of ginsenoside-Ro on cell proliferation and cytokine production in murine splenocytes. Methods The effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3 H] thymidine incorporation assay. Effects of ginsenoside-Ro on the production of cytokines interleukin-2 (IL-2), interferon-γ (IFN-γ) and interleukin-4 (IL-4) from murine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of Th1 cytokine IFN-γ and Th2cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction (RT-PCR) analysis.Results Ginsenoside-Ro showed no mitogenic effect on unstimulated murine splenocytes. It enhanced the proliferation of Con A-induced murine splenocytes and the production of IL-2 at concentrations of 1 - 10decreased the production and expression of Th1 cytokine IFN-γ in Con A-induced murine splenocytes at regulating the production and expression of Th1/Th2 cytokines in murine splenocytes.%目的研究人参皂苷-Ro对小鼠脾细胞增殖及细胞因子产生的影响.方法 [3H]TdR参入法检测人参皂苷-Ro对小鼠脾淋巴细胞增殖的影响;酶联免疫吸附法检测人参皂苷-Ro对小鼠脾淋巴细胞产生细胞因子白介素-2、干扰素-γ和白介素-4的影响;逆转录聚合酶链式反应分析法研究人参皂苷-Ro对小鼠脾淋巴细胞中干扰素-γ、白介素-4mRNA表达的影响.结果人参皂苷-Ro在1-10μmol·L-1显著促进Con A诱导的小鼠脾淋巴细胞增殖及小鼠脾淋巴细胞白介素-2的产生;在2-10 μmol·L-1促进Con A诱导的小鼠脾淋巴细胞产生和表达Th2细胞因子白介素-4,而降低Con A诱导的小鼠脾淋巴细胞产生和表达Th1细胞因子干扰素-γ.结论人参皂苷-Ro通过调节脾细胞内Th1型和Th2型细胞因子的转录和表达发挥免疫调节作用.
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