Whole-genome sequencing and analysis of the Chinese herbal plant Gelsemium elegans

Yisong Liu; Qi Tang; Pi Cheng; Mingfei Zhu; Hui Zhang; Jiazhe Liu; Mengting Zuo; Chongyin Huang; Changqiao Wu; Zhiliang Sun; Zhaoying Liu

首页> 中文期刊> 《药学学报：英文版》 >Whole-genome sequencing and analysis of the Chinese herbal plant Gelsemium elegans

Whole-genome sequencing and analysis of the Chinese herbal plant Gelsemium elegans

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Gelsemium elegans(G.elegans)(2 n=2 x=16)is genus of flowering plants belonging to the Gelsemicaeae family.Here,a high-quality genome assembly using the Oxford Nanopore Technologies(ONT)platform and high-throughput chromosome conformation capture techniques(Hi-C)were used.A total of 56.11 Gb of raw GridION X5 platform ONT reads(6.23 Gb per cell)were generated.After filtering,53.45 Gb of clean reads were obtained,giving 160 x coverage depth.The de novo genome assemblies 335.13 Mb,close to the 338 Mb estimated by k-mer analysis,was generated with contig N50 of 10.23 Mb.The vast majority(99.2%)of the G.elegans assembled sequence was anchored onto 8 pseudo-chromosomes.The genome completeness was then evaluated and 1338 of the 1440 conserved genes(92.9%)could be found in the assembly.Genome annotation revealed that 43.16%of the G.elegans genome is composed of repetitive elements and 23.9%is composed of long terminal repeat elements.We predicted 26,768 protein-coding genes,of which 84.56%were functionally annotated.The genomic sequences of G.elegans could be a valuable source for comparative genomic analysis in the Gelsemicaeae family and will be useful for understanding the phylogenetic relationships of the indole alkaloid metabolism.

著录项

来源
《药学学报：英文版》 |2020年第2期|P.374-382|共9页
作者
Yisong Liu; Qi Tang; Pi Cheng; Mingfei Zhu; Hui Zhang; Jiazhe Liu; Mengting Zuo; Chongyin Huang; Changqiao Wu; Zhiliang Sun; Zhaoying Liu;
展开▼
作者单位

Hunan Engineering Technology Research Center of Veterinary Drugs Hunan Agricultural University Changsha 410128 ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine Hunan Agricultural University Changsha 410128 ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha 410128 China;

Hunan Key Laboratory of Traditional Chinese Veterinary Medicine Hunan Agricultural University Changsha 410128 China;

Hunan Key Laboratory of Traditional Chinese Veterinary Medicine Hunan Agricultural University Changsha 410128 China;

Nextomics Biosciences Institute Wuhan 430000 China;

Nextomics Biosciences Institute Wuhan 430000 China;

Nextomics Biosciences Institute Wuhan 430000 China;

College of Veterinary Medicine Hunan Agricultural University Changsha 410128 China;

College of Veterinary Medicine Hunan Agricultural University Changsha 410128 China;

Hunan Key Laboratory of Traditional Chinese Veterinary Medicine Hunan Agricultural University Changsha 410128 China;

Hunan Engineering Technology Research Center of Veterinary Drugs Hunan Agricultural University Changsha 410128 ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine Hunan Agricultural University Changsha 410128 ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha 410128 China;

Hunan Engineering Technology Research Center of Veterinary Drugs Hunan Agricultural University Changsha 410128 ChinaHunan Key Laboratory of Traditional Chinese Veterinary Medicine Hunan Agricultural University Changsha 410128 ChinaCollege of Veterinary Medicine Hunan Agricultural University Changsha 410128 China;

展开▼
原文格式 PDF
正文语种 chi
中图分类 S567.19;
关键词
Gelsemium elegans; Nanopore sequencing; Genome assembly; Hi-C; Genome annotation; Monoterpene indole alkaloid;

机译：凝胶杆菌;纳米孔测序;基因组组装;HI-C;基因组注释;单点吲哚生物碱;

Whole-genome sequencing and analysis of the Chinese herbal plant Gelsemium elegans

摘要

著录项

相关主题

期刊订阅