Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis

Chao TANG; An-hua WU; Hong-li XUE; Yun-jie WANG

首页> 外文期刊>中国药理学报：英文版 >Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis

【24h】

Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis

机译：丹参酮IIA通过抑制内皮素转化酶1的合成来抑制TNF-α诱导的脑微血管内皮细胞中内皮素1的产生

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Aim: To investigate the effects of tanshinone ⅡA (Tan ⅡA) on the regulation of the production of endothelin (ET)-1 (including large ET-1), mRNA levels of ET-1,endothelin-converting enzyme- 1 (ECE- 1), endothelin-A receptor (ETA) and endothelin-B receptor (ETB) induced by TNF-α in rat brain microvascular endo-thelial cells (BMVEC). Methods: The ET-1 release (including large ET-1) into the culture medium was determined by enzyme immunoassay. The levels of ET-1,ECE- 1, ETA, and ETB mRNA were measured by RT-PCR. Endothelin receptor bind-ing was also tested. Results: The induction of ET- 1 release by TNF-α from cul-tured BMVEC was dose-dependently reduced by Tan IIA, but large ET-1 levels progressively increased in response to Tan IIA; the mRNA expression of ET-1 was unaffected. Tan ⅡA also caused a decrease in ETA receptor mRNA and ECE-1expression in a dose-dependent manner. Endothelin receptor binding was unal-tered in BMVEC stimulated with TNF-α alone or a combination of TNF-α and Tan ⅡA. Conclusion: These findings suggest that Tan ⅡA may inhibit ET-1 produc-tion in TNF-α-induced BMVEC through the suppression of ECE-1 synthesis.

机译：目的：探讨丹参酮ⅡA（TanⅡA）对内皮素（ET）-1（包括大ET-1）的产生，ET-1 mRNA水平，内皮素转化酶-1（ECE- 1），TNF-α在大鼠脑微血管内皮细胞（BMVEC）中诱导的内皮素A受体（ETA）和内皮素B受体（ETB）。方法：采用酶联免疫法测定ET-1（包括大ET-1）向培养基中的释放。通过RT-PCR测量ET-1，ECE-1，ETA和ETB mRNA的水平。还测试了内皮素受体结合。结果：Tan IIA剂量依赖性地降低了培养的BMVEC中TNF-α释放ET-1的诱导，但对Tan IIA的反应，大的ET-1水平逐渐升高。 ET-1的mRNA表达不受影响。 TanⅡA也引起ETA受体mRNA和ECE-1表达的降低，且呈剂量依赖性。在单独用TNF-α或TNF-α和TanⅡA联合刺激的BMVEC中，内皮素受体结合没有改变。结论：这些发现提示，TanⅡA可能通过抑制ECE-1的合成而抑制TNF-α诱导的BMVEC中ET-1的产生。

著录项

来源
《中国药理学报：英文版》 |2007年第8期|1116-1122|共7页
作者
Chao TANG; An-hua WU; Hong-li XUE; Yun-jie WANG;
展开▼
作者单位

Department of Neurosurgery, the First Affiliated Hospital, China Medical University;

Department of Neurosurgery, the First Affiliated Hospital, China Medical University;

Department of Neurosurgery, General Hospital oJ Shenyang Military Command, Shenyang 110001,China.;

Department of Neurosurgery, the First Affiliated Hospital, China Medical University;

展开▼
收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 chi
中图分类药学;
关键词
tanshinone ⅡA; endothelin-1; TNF-α; brain microvascular endothelial cells;

机译：丹参酮ⅡA;内皮素-1;TNF-α;脑微血管内皮细胞;

Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis

摘要

著录项

相关主题

期刊订阅