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首页> 外文期刊>中国药理学报：英文版 >Influence of connective tissue growth factor antisense oligonucleotide on angiotensin Ⅱ-induced epithelial mesenchymal transition in HK2 cells
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Influence of connective tissue growth factor antisense oligonucleotide on angiotensin Ⅱ-induced epithelial mesenchymal transition in HK2 cells

机译：结缔组织生长因子反义寡核苷酸对血管紧张素Ⅱ诱导的HK2细胞上皮间质转化的影响

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摘要

Aim: The present study was designed to further investigate the effect of connective tissue growth factor antisense oligonucleotide (CTGF-AS) on angiotensin Ⅱ (Ang Ⅱ)-induced tubular cell epithelial mesenchymal transition (EMT) in vitro. Methods: The human proximal tubular cell line (HK2) was grown in Dulbecco's modified Eagle's medium containing 10% heat inactivated fetal calf serum. After being rested in serum-free medium for 24 h, the influence of CTGF-AS (20 μg/mL) on Ang Ⅱ-induced (1×10-7 mol/L) CTGF mRNA and the protein expression were examined by using reverse transcription-polymerase chain reaction and indirectimmunofluorescence. The effect of CTGF-AS on Ang Ⅱ-induced cellular ultrastructure was observed using a transmissive electronic microscope. The expression of α-smooth action (α-SMA) was assayed by immunocytochemistry. In all experiments, the control group was treated with scrambled oligonucleotide. Results: It was shown that Ang Ⅱ significantly induced the increasing expression of CTGF mRNA and protein (P＜0.01, respectively), which were significantly abolished by treatment with CTGF-AS. After stimulating cells with Ang Ⅱ, the cellular ultrastructure showed mesenchymal features. These effects were partially inhibited by CTGF-AS. Ang Ⅱ significantly resulted in the expression of α-SMA in time dependent manner, which was markedly attenuated by the treatment with CTGF-AS (P＜0.01, respectively). In contrast, no similar effects were observed in the control group treated with scrambled oligonucleotide. Conclusion: Ang Ⅱ-induced EMT in human proximal tubular epithelial cells (PTC) can be attenuated by treatment with CTGF-AS. Our data provides further evidence that CTGF might be involved in Ang Ⅱ-induced EMT in PTC.
机译：目的：本研究旨在进一步研究结缔组织生长因子反义寡核苷酸（CTGF-AS）对血管紧张素Ⅱ（ANGⅡ）诱导的管状细胞上皮间充质转换（EMT）的影响。方法：人近端管细胞系（HK2）在含有10％热灭活胎牛血清的Dulbecco改性鹰培养基中生长。通过使用反向检查在24小时内静置24小时24小时，通过反向检查CT​​GF-AS（20μg/ ml）对抗Ⅱ诱导的（1×10-7mol / L）CTGF mRNA和蛋白质表达后的影响转录聚合酶链反应和间接免疫荧光。使用透射电子显微镜观察CTGF-如在AngⅡ诱导的细胞超微结构的影响。通过免疫细胞化学测定α-平滑作用（α-SMA）的表达。在所有实验中，对照组用丙酸寡核苷酸处理。结果：显示AngⅡ显着诱导CTGF mRNA和蛋白质的增加（分别分别为P <0.01），这通过CTGF处理显着废除。在用AngⅡ刺激细胞后，细胞超微结构显示间充质特征。 CTGF-AS部分抑制这些效果。 AngⅡ显着导致α-SMA的表达，随着时间的依赖性方式表达，这通过CTGF的处理显着减弱（分别为P <0.01）。相反，在用糖核核苷酸处理的对照组中没有观察到类似的效果。结论：通过用CTGF-AS处理可以衰减ANGⅡ诱导的人近端上皮细胞（PTC）中的EMT。我们的数据提供了进一步的证据表明CTGF可能参与PTC中的AngⅡ诱导的EMT。

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  • 来源
    《中国药理学报：英文版》 |2006年第8期|1029-1036|共8页
  • 作者

    Long CHEN; Bi-cheng LIU; Xiao-liang ZHANG; Jian-dong ZHANG; Hong LIU; Min-xia LI;

  • 作者单位

    Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing 210009, China;

    Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing 210009, China;

    Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing 210009, China;

    Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing 210009, China;

    Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing 210009, China;

    Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing 210009, China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 药学;
  • 关键词

    connective tissue growth factor; angiotensin Ⅱ; epithelial mesenchymal transition; proximal kidney tubules; humans;

    机译：结缔组织生长因子;血管紧张素Ⅱ;上皮间质转化;近端肾小管;人;
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