Chemical and pharmacological evaluation of Hypericum perforatum extracts

B Duff SLOLEY; Liana J URICHUK; LING Lei; GU Lie-Dong; Ronald T COUTrSa; Peter K T PANG; Jacqueline J SHAN

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Chemical and pharmacological evaluation of Hypericum perforatum extracts

机译：贯叶连翘提取物的化学和药理评价

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AIM: To determine the concentrations of chemical characteristic to extracts of leaves and flowers of Hypericum perforatum (St John's wort) in a number of selected samples and, following chemical characterization, to investigate the effects of these extracts on several pharmacological properties including effects of the extracts on inhibition of 5-hydroxytryptamine (5-HT) uptake and on antioxidant properties. METHODS: The samples were analyzed for the presence of characteristic chemicals by high performance liquid chromatography (HPLC) directly coupled to ultraviolet wavelength absorbance and positive or negative mode electrospray mass spectrometric detection. The effects of extracts on 5-HT uptake were determined by quantifying 3H-5-HT incorporation into rat hippocampal prisms. Estimates of effects of extracts on fire radical scavenging capacity were made using a dynamic assay based on the ability of compounds to prevent the initiation of a colored reaction produced by the horseradish peroxidase catalyzed formation of hydroxyl free radicals from hydrogen peroxide using 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) as the color indicator. RESULTS: The chemical profile of a number of extracts were determined and found to differ substantially from each other. Inhibition of 5-HT uptake was found to correlate with hyperforin content and free radical scavenging capacity was found to correlate with the content of several flavonoids including quercetin and hyperoside.CONCLUSION: Standardized extracts of H perforatum varied substantially in the concentration of several characteristic chemicals. The correlation between pharmacological activity and certain characteristic chemicals found in these extracts indicates that the medicinal benefit derived from selected extracts will vary considerably depending on their chemical composition.

机译：目的：确定一些选定样品中贯叶连翘（Sophia perforatum）（圣约翰草）的叶和花提取物的化学特征浓度，并根据化学表征，研究这些提取物对几种药理特性的影响，包括提取物对5-羟色胺（5-HT）摄取的抑制作用和抗氧化性能。方法：采用高效液相色谱法（HPLC）直接结合紫外波长吸收和正或负模式电喷雾质谱检测，分析样品中是否存在特征性化学物质。通过量化3H-5-HT掺入大鼠海马棱柱中来确定提取物对5-HT摄取的影响。基于化合物防止由辣根过氧化物酶催化的过氧化氢形成羟基自由基产生的有色反应的引发能力的动态测定，使用动态测定法估算了提取物对火自由基清除能力的影响，使用2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）作为颜色指示剂。结果：确定了许多提取物的化学特征，发现它们之间存在显着差异。研究发现5-HT的抑制作用与高forin的含量有关，而自由基清除能力与槲皮素和hyperoside等几种类黄酮的含量有关。在这些提取物中发现的药理活性与某些特征化学物质之间的相关性表明，从选定提取物中获得的医学益处将根据其化学组成而有很大不同。

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来源
《中国药理学报：英文版》 |2000年第12期|1145-1152|共8页
作者
B Duff SLOLEY; Liana J URICHUK; LING Lei; GU Lie-Dong; Ronald T COUTrSa; Peter K T PANG; Jacqueline J SHAN;
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作者单位

CV Technologies, Edmonton Research Park, 9411 - 20 Ave, Edmonton, Alberta, Canada T6N 1E5;

CV Technologies, Edmonton Research Park, 9411 - 20 Ave, Edmonton, Alberta, Canada T6N 1E5;

CV Technologies, Edmonton Research Park, 9411 - 20 Ave, Edmonton, Alberta, Canada T6N 1E5;

CV Technologies, Edmonton Research Park, 9411 - 20 Ave, Edmonton, Alberta, Canada T6N 1E5;

Neurochemical Research Unit, Department of Psychiatry, University of Alberta, Edmonton, Alberta, Canada T6G 2B7, Canada;

CV Technologies, Edmonton Research Park, 9411 - 20 Ave, Edmonton, Alberta, Canada T6N 1E5;

CV Technologies, Edmonton Research Park, 9411 - 20 Ave, Edmonton, Alberta, Canada T6N 1E5;

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收录信息北京大学中文核心期刊目录(北大核心);中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 chi
中图分类
关键词
Hypericum perforatum; High pressure liquid chromatography; serotonin uptake inhibitors; free radicals; antioxidants; hyperforin; hypericin; quercetin; mtin;

机译：贯叶连翘;高压液相色谱法;5-羟色胺摄取抑制剂;自由基;抗氧化剂;Hyperforin;Hypericin;槲皮素;mtin;

Chemical and pharmacological evaluation of Hypericum perforatum extracts

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