采用盆栽模拟试验,通过豌豆与玉米间作,并对单作和间作豌豆进行根瘤菌接种,研究了接种对豌豆结瘤固氮特性、豌豆及间作玉米的氮素吸收量、作物生长状况的影响.结果表明,间作及接种提高了固氮酶的活性;相对于间作不接种,间作接种根瘤菌ACCC16101(R1)和XC3.1 (R2)后,根瘤重量增加了19.0%~120.4%;ACCC16101对间作作物的氮素吸收量及干物质积累量影响较小;间作接种XC3.1后,豌豆、玉米的氮素吸收量及干物质积累量显著增加,籽粒产量比间作不接种提高16.9%和19.1%,土地当量比也高于接种ACCC16101和不接种;XC3.1适宜在豌豆/玉米间作系统中接种.%A Rhizobium inoculation experiment was conducted in a pea/maize intercropping system to investigate the changes of nodulation, nitrogen (N) fixation, N absorption, and crop growth. Nitrogenase activity of Rhizobium in pea nodules was increased significantly by intercropping and inoculation with ACCC16101 (R1) and XC3. 1 (R2). Nodule weights of intercropped pea increased by 19. 0% - 120. 4% after inoculation with these two Rhizobium strains. ACCC16101 had little effect on N absorption and dry matter accumulation of either pea or maize. In an intercropping system inoculated with XC3. 1, N and dry matter accumulation of these two crops were considerably improved and seed yields of pea and maize increased by 16. 9% and 19.1% , respectively. XC3. 1 is suitable for inoculation in a pea/maize system.
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