通过培养皿发芽试验,从127株烟草内生细菌中初步筛选出4株能提高烟草种子发芽率、根长、根体积的内生细菌.对这4个菌株进行了漂浮育苗试验复筛,发现它们对烟苗的生长具有明显促进作用.其中促生作用最好的wy2菌株使烟苗茎直径增加了33.3％,根长增加了49.2％,与对照相比差异显著.通过16S rDNA序列同源性分析将wy2菌株鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens).%Four growth-promoting bacteria were screened out from 127 endophytic bacteria based on germination rate, roots length and volume of tobacco seeds in culture dish experiments. Further screening was carried out in floating seedling production. Results indicated that the best strain wy2 significantly promoted stem diameter by 33.3%, root length by 49.2%. Wy2 was identified as Bacillus amyloliquefaciens according to its 16S rDNA sequence.
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