目的 研究芹菜素对肥胖大鼠肾脏代谢状态的影响以及可能机制.方法 4周龄雄性SD大鼠按随机数字表法分为对照组和高脂组.高脂组予连续高脂喂养12 周后,再随机分为肥胖组、干预组.对照组和肥胖组予0.9%生理盐水灌胃,干预组予芹菜素40 mg/(kg·d)灌胃处理,4周后通过Western blot、DHE探针染色和免疫组化检测肾脏组织中氧化应激、炎症和自噬相关指标.结果 ① 芹菜素干预4周后,与肥胖组相比,芹菜素组体重有一定的下降趋势,但是差异无统计学意义(t=1.80,P=0.096).② DHE探针染色检测肥胖大鼠肾脏示O2-较对照组明显增多( t= -3.58,P=0.023),经过4 周菜素治疗后明显好转(t =2.79, P =0.049).③ 肥胖大鼠肾脏p-P67、p-P65等表达明显增加,芹菜素干预后,其蛋白水平较肥胖组(均P＜0.05)出现显著下降.④ 经芹菜素治疗后肾脏自噬相关蛋白LC3BII/LC3BI、Beclin1水平明显上升,与肥胖组相比差异有统计学意义(均P＜0.05).结论 芹菜素可明显改善肥胖大鼠肾脏内氧化应激、炎症的代谢紊乱状态,可能与其激活自噬通路有关.%Objective To investigate the effects of apigenin on renal metabolic status and its possible mechanism in obese rats. Methods All 4-week-oldmale SD rats were randomly divided into Con group and HFD group. The HFD group was fed with high-fat diet for 12 weeks, then divided into Apigenin group and Obesity group randomly. The Apigenin group wastreated with apigenin 40 mg/( kg·d) for another 4 weeks. The Con and Obesity groups of rats were received 0.9% normal saline in the meantime. Western blot and immunohistochemical were used for pro-tein expression assays. Results ① After 4 weeks apigenin treatment, the rat weights were decreased compared with Obesity group(t=1.80,P=0.096), but the difference was not statistically significant. ②DHE staining was used to evaluate the O2- formation in the kidney. In apigenin group, the content of O2- was decreased distinctly compared with Obesity(t=2.79,P=0.049). ③ The protein expression of p-P67 and p-p65 were decreased com-pared with Obesity (P＜0.05) after apigenin treatment. ④ LC3BII/LC3BI and Beclin1 protein levels in kidney were increased significantly compared with the other two groups after apigenin treatment(P＜0.05). Conclusion Apigenin alleviates the obese rats renal oxidative stress and inflammation state, which may be related to the activa-tion of autophagy pathway.
展开▼
