10 single-cell clones of HepG2.2.15 cells were isolated by limited dilution and their expressions of HBsAg and HBeAg were determined by ELISA.According to expressions of HBsAg and HBeAg, the highest, medium and the lowest-expression clones were selected.Cytotoxicity of IFN-α were analyzed by MTT assay and the results suggested that IFN-α showed similar cytotoxicity to the different clonal cell lines.The clones were treated with IFN-α and HBsAg and HBeAg expressions were determined by ELISA.IFN-α weakly inhibited HBeAg expression and strongly inhibited HBsAg expression and HBV DNA replication, and the inhibition effect was stronger for those with higher expression levels.HBsAg and HBeAg were differentially expressed in HepG2.2.15 cells and the highly expressed clones were more sensitive to IFN-α.%采用有限稀释法从HepG2.2.15细胞株中分离出10株单克隆细胞株.ELISA法检测其HBsAg与HBeAg表达量,根据HBsAg、HBeAg表达量的不同,筛选出最高、中等、最低3株克隆细胞株.MTT法进行干扰素(IFN)-α的细胞毒作用研究,表明IFN-α对不同克隆细胞株药物毒作用相似.ELISA法检测各克隆细胞株给IFN-α前后HBsAg、HBeAg表达量,IFN-α对HepG2.2.15细胞HBeAg的抑制作用较弱,对HBsAg、HBV DNA的抑制作用较强,且对表达量高的克隆细胞株抑制作用更强.不同HepG2.2.15细胞表达HBsAg与HBeAg存在差异性,且表达量高的细胞株对治疗浓度的IFN-α更加敏感.
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