目的:探讨微小RNA(miR)-200a的过表达对结直肠癌SW1116细胞迁移和侵袭能力的影响。方法利用Lipo-fectamineTM 2000瞬时转染含miR-200a的质粒,实验组将载有miR-200a的质粒转染至结直肠癌SW1116细胞中,细胞对照组结直肠癌SW1116细胞不做任何处理;应用实时定量PCR法分析miR-200a的表达变化,划痕实验检测细胞迁移能力的改变、Transwell小室侵袭实验检测细胞侵袭能力的改变。结果实时定量PCR结果表明,与细胞对照组比较,实验组经瞬时转染后miR-200a的表达水平显著升高( P<0.01);划痕实验和Transwell小室侵袭实验表明外源性过表达miR-200a可促进结直肠癌SW1116细胞的迁移和侵袭能力,与细胞对照组比较差异均有统计学意义(P<0.01)。结论 miR-200a 能明显促进结直肠癌SW1116细胞的侵袭和迁移。%Objective To investigate the effect of microRNA ( miR)-200a on migration and invasion abilities of colorectal cancer SW1116 cells.Methods miR-200a plasmid was transfected into SW1116 cells in colorectal cancer SW1116 cells by Lipofectamine TM 2000 as the experimental group , colorectal cancer SW1116 cells in the control group do not do any processing .The expression of miR-200 a in the experimental group was confirmed by re-al-time PCR.The migration abilities of cells were detected by Transwell invasion assay ,and the invasion abilities of cells were detected by wound-healing assay .Results Real-time quantitative PCR results showed that compared with the control group ,experimental group after transient transfection of miR-200 a was significantly increased ( P<0.01).Exogenous overexpression of miR-200a could promote migration and invasion abilities of SW 1116 cells,com-pared with the control group the difference was statistically significant (P<0.01).Conclusion miR-200a can promote the migration and invasion abilities of colorectal cancer SW 1116 cells.
展开▼