目的：探讨α-硫辛酸(α-LA)对H9c2心肌细胞低氧及低氧/复氧损伤的保护作用及其作用机制。方法研究包括H9 c2心肌细胞低氧实验和低氧/复氧实验两部分,分成常氧组、低氧组或低氧/复氧组、LA组、乙醛脱氢酶抑制剂异黄酮苷(Daidzin)组(Daidzin组)、二甲亚砜(DMSO)溶剂对照组( DMSO组)。采用噻唑蓝( MTT)比色法检测心肌细胞存活率；微量酶标法测定乳酸脱氢酶( LDH)活性；ELISA法检测心肌细胞乙醛脱氢酶2(ALDH2)活性；硫代苯巴比妥酸法检测丙二醛( MDA)水平。结果与常氧组比较,低氧组及低氧/复氧组细胞存活率均下降(P0．05)；Daid-zin组与LA组、DMSO组比较,细胞存活率及ALDH2活性降低(P0. 05 ) . Conclusion α-LA can protect H9 c2 cardiomyocytes from hypoxia or H/R injury by means of upregulating activities of ALDH2 and decreasing hypoxia or H/R induced lipid peroxidation.
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