Objective To investigate the association between TLR4,C-Jun N-terminal kinase,tumor necrosis factor-α(TNF-α),Leptin changes and chronic intermittent hypoxia(CIH). Methods Twenty-four SD rats were divided into 3 groups (n=8):control group,CIH 6 weeks group, CIH 8 weeks group. After the modeling,the change of blood pressure and renal artery were observed by the use of non-invasive blood pressure analyzer and HE staining. ELISA was employed to determine the production of TNF-αand Leptin in sera of these animals. The expressions of TLR4 and JNK mRNA were detected by real time quantitativer everse transcription-polymerase chain reaction ( QRT-PCR) method in the adipose tissue. Results The blood pressure in intermittent hypoxia group was signifi-cantly higher than those in control group(P<0.01), and the renal artery pathological changes in intermittent hy-poxia group were significantly heavier than those in control group;the content of TNF-α and Leptin in intermittent hypoxia was significantly higher than those in control group(P<0.01),and the content of TNF-α and Leptin was significantly increased with the days of hypoxia;the expression of TLR4 and JNK at mRNA levels in intermittent hy-poxia was significantly higher than those in control group(P<0.01),and the expression of TLR4 and JNK at mR-NA levels was significantly increased with the days of hypoxia. Conclusion TLR4,JNK,TNF-α,Leptin may be in-volved in the inflammatory response of chronic intermittent hypoxia in rats.%目的探讨Toll样受体4( TLR4)、c-Jun氨基末端激酶( JNK)、肿瘤坏死因子α( TNF-α)、瘦素( Leptin)与慢性间歇性缺氧( CIH)的关系。方法将24只SD大鼠随机分成3组：对照组、CIH 6周组、CIH 8周组,每组8只。造模成功后,采用无创血压分析仪观察大鼠血压变化,HE染色观察肾动脉病理学改变,采用ELISA法检测血清TNF-α、Leptin的含量,利用实时荧光定量 PCR 技术检测大鼠脂肪组织中TLR4、JNK mRNA表达水平。结果模型组大鼠血压明显高于对照组(P<0.01),且模型组大鼠肾动脉病理变化较对照组显著加重；CIH 6周组和CIH 8周组的血清TNF-α、Lep-tin的含量与对照组比较显著增加( P <0.01),且 TNF-α、Leptin的含量随缺氧天数的增加而显著增加( P <0.01)；CIH 6周组和CIH 8周组的TLR4、JNK mRNA水平比对照组显著增加( P<0.01),且TLR4、JNK mRNA水平随缺氧天数的增加而显著增加( P<0.01)。结论TLR4、JNK、TNF-α、Leptin可能参与了CIH大鼠的炎症反应。
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