目的 探讨胃癌患者外周血及胃癌组织中CD4+CD25+调节性T细胞(Tregs)和转录因子Foxp3表达及临床意义.方法 收集38例胃癌患者术前血液样本和20例健康体检者血液样本进行流式细胞仪检测,同时胃癌组中留取胃癌组织和癌旁组织(距肿瘤边缘>5 cm)进行免疫组化染色,检测CD4+CD25+Foxp3+Tregs表达,并与胃癌的TNM分期、肿瘤分化程度、淋巴转移、肿瘤所在部位、肿瘤直径等相关参数进行分析.结果 胃癌组外周血中CD4+CD25+Foxp3+Tregs的表达水平与对照组相比差异有统计学意义(P＜0.01),且在胃癌TNM分期、淋巴转移组内比较差异有统计学意义(P＜0.01).胃癌组织中Foxp3+Tregs的表达水平与癌旁组织相比,差异有统计学意义(P＜0.01),且在胃癌TNM分期、分化程度、淋巴转移组内比较差异有统计学意义(P＜0.01,P＜0.05).外周血中CD4+CD25+ Foxp3+Tregs与胃癌组织中Foxp3+Tregs的表达水平呈显著正相关(r=0.786,P 5 cm ) from gastric cancer were subjected to immunohis-tochemistry to detect CD4 + CD25 + Foxp3 + Tregs expression, and the correlation analysis with some clinical related parameters was made. Results The percentages of CD4 + CD25 + Foxp3 + Tregs in peripheral blood of gastric cancer group were significantly higher than those from healthy control group( P <0. 01 ),and were highly correlated to TNM stage, lymph node metastases( P <0. 01 ). The percentages of Foxp3 + Tregs in gastric cancer tissue were significantly higher than those from para-tumorous tissue( P <0. 01 ),and were highly correlated to TNM stage,tumor differentiation,lymph node metastases( P < 0. 01 , P < 0. 05 ). The percentages of CD4 + CD25 + Foxp3 + Tregs in peripheral blood was positively correlated to Foxp3 + Tregs in gastric cancer tissue( r - 0. 786, P < 0. 01 ). Conclusion The proportion of CD4 + CD25 + Foxp3 + Tregs in Peripheral blood and Foxp3 + Tregs in gastric cancer tissue is significantly elevated in human gastric cancer, and correlated with the genesis and development of gastric cancer, which can provide the theory basis for the early diagnosis and immunotherapy of gastric cancer.
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