目的 检测并分析肾癌(透明细胞癌)组织中HDAC7 mRNA和蛋白的表达差异,探讨其与肾癌的发生、发展以及患者预后之间的关系.方法 应用实时荧光定量PCR和免疫组化法对60 例肾癌组织及20例正常肾组织中HDAC7的表达情况进行检测,并通过对患者随访,探讨其蛋白表达水平与肾癌临床病理学指标及患者预后之间的关系.结果 HDAC7在肾癌组织中的表达较正常肾组织明显增高,且与肾癌的病理分级和临床分期之间均呈负相关(P＜0.05).HDAC7低表达者的生存率低于HDAC7高表达者(P＜0.05).结论 HDAC7可能与肾癌的发生、发展有关,提示HDAC7可作为评估肾癌恶性程度及预后的肿瘤标志物.%Objective To investigate the mRNA and protein expression of HDAC 7 in renal cell carcinoma, and to study the clinical value of protein level in the occurrence , progression of renal cell carcinoma ( RCC). Methods 60 cases RCC and 20 normal renal tissue specimens had been detected by real time fluorescence quantitative PCR and immunohistochemical methods. Then we analyzed the relationship between HDAC 7 protein level and clinical parameters as well as the prognosis of patients . Results The expression of HDAC 7 was higher in RCC than that in normal renal tissue. There were significantly positive correlation between the expression of HDAC 7 protein and the pathological grades/stages of rRCC ( P < 0.05 ). The survival rate of these patients whose low expression of HDAC 7 protein was lower than those of high expression of HDAC 7 protein. Conclusion There may be relationship between the expression of HDAC 7 protein and the occurrence , progression of RCC. HDAC 7 can be used as a tumor marker for the evaluation of malignant potential and prognosis of RCC .
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