目的 探讨肝细胞生长因子(HGF)在浸润性乳腺癌中的表达及其对新辅助化疗敏感性和预后的影响.方法 免疫组化法检测125例浸润性乳腺癌组织中HGF的表达,分析HGF与患者术前化疗及5年生存率的关系;利用si-RNA沉默乳腺癌细胞株MCF-7检测 HGF的表达,MTT法检测加入不同浓度表阿霉素共培养后的细胞增殖能力.结果 HGF阳性组与阴性组在TNM分期、组织学分级、淋巴结转移、新辅助化疗疗效及预后方面差异有统计学意义(P＜0.05),下调HGF后的MCF-7细胞对表阿霉素的耐药性减弱.结论 HGF在乳腺癌中表达与淋巴结转移、预后及化疗敏感性密切相关,有可能作为判断预后和预测化疗疗效的指标之一.%Objective To study the expression of hepatocyte growth factor( HGF ) in tissue and cells in patients with invasive breast cancer and the effects of HGF on neoadjuvant chemotherapy and prognosis of the patients. Methods Immunohistochemical method was used to detect the expression of HGF in the paraffin section of 125 cases of invasive breast cancer. The relationship of the HGF expression, preoperative chemotherapy and five years' survival rate was analyzed. The expression of HGF was detected by si-RNA MCF-7. The cell proliferation of breast cancer cells co-cultured with different concentrations of doxorubicin was determined by MTT. Results The expression of HGF between negative and positive group was significant difference in the histological grade, TNM stage, lymphonodus metastasis, chemotherapy effect and prognosis ( P <0. 05 ). The drug resistance of cells to doxorubicin was weakened after down-regulation of the level of HGF. Conclusion HGF is strongly associated with lymphonodus metastasis, prognosis and chemosensitivity in breast neoplasm, which may be used as one of the independent parameters to the prognostic and the effect of chemotherapy.
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