目的 探讨对氧磷酶1(PON1)192和55位点的基因多态性与2型糖尿病视网膜病变(DR)关系.方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测184例不同程度视网膜病变的2型糖尿病(T2DM)患者和136例健康对照者(NGT) PON1 Q192R和L55M的多态性.结果 PON1Q192R基因在DR组QQ基因型及Q等位基因频率高于NDR组,差异有统计学意义(P<0.05).但NGT与T2DM比较以及非增殖期糖尿病视网膜病变(NPDR)与增殖期糖尿病视网膜病变(PDR)比较,差异无统计学意义.PON1L55M基因在各组间的分布差异无统计学意义.结论 PON1Q192R基因多态性可能与DR的发生有关,但与DR的严重程度无关,Q等位基因是DR的独立危险因素,PON1L55M基因多态性与DR无显著相关性.%Objective To investigate the correlation between paraoxionase gene polymorphisms ( Q192R and L55M ) and type 2 diabetes mellitus retinopathy. Methods Paraoxionase 1 gene polymorphisms ( Q192R and L55M ) were determined by PCR-RFLP in 184 type 2 diabetes patients with various degree of retinopathy and 136 normal control subjects. Results It had significant difference between NDR and DR in Q192R gene, QQ and the rate of Q allelic genes ( P < 0. 05 ), but no difference between NGT and T2DM, also no difference between NPDR and PDR. The distribution of PON1L55M gene had no significant differences in each group. Conclusion PON1 Q192R is significantly associated with retinopathy in type 2 diabetes,but it is not associated with the progression of DR, Q allele appears to be an independent risk factor of type 2 diabetes retinopathy. PON1 L55M has no association with retinopathy in type 2 diabetes mellitus.
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