Objective To investigate the expression of Twist,MMP-2 and MMP-9 protein and their relationships in breast cancer. Methods The expression of Twist,MMP-2 and MMP-9 protein was examinated with tissue chips in 159 cases of breast cancer by SP immunohistochemical method. Results The positive rates of Twist, MMP-2 and MMP-9 protein were 73. 8％ ,96. 9％ and 95. 0％ , respectively. The high expression of Twist was positively correlated to axillary lymph node metastasis and TNM staging( P ＜ 0. 01 ). The patients’ overall survival and relapse-free survival were shorter in the group of Twist high expression than that in the group of Twist low expression( P ＜0. 05 ). Both the expression of MMP-2 and MMP-9 were positively correlated to Twist expression( P ＜0. 01 ). Conclusion The expression of Twist might be closely correlated to the tumor invasion, metastasis, patients' prognosis and MMP-2 and MMP-9 in breast cancer.%目的 研究Twist和明胶酶(MMP-2和MMP-9)蛋白在乳腺癌组织中的表达及其相互关系.方法 建立组织芯片平台,应用免疫组化SP法检测159例乳腺癌组织Twist、MMP-2和MMP-9蛋白的表达情况.结果 乳腺癌中Twist的高表达率为73.8%,MMP-2和MMP-9阳性率分别为96.9%和95.0%; Twist的表达与乳腺癌腋淋巴结受累、TNM分期均呈正相关(P<0.01);Twist高表达患者总生存期和无复发生存期显著低于低表达患者(P<0.01,P<0.05);MMP-2、MMP-9蛋白表达与Twist表达均呈正相关(P<0.01).结论 乳腺癌中Twist表达与肿瘤侵袭转移、患者预后有密切关系,Twist可能在一定水平上调控明胶酶表达.
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