目的:通过比较电针心经和肺经腧穴对急性心肌缺血(AMI)大鼠心肌组织超极化激活的环核苷酸门控通道2(HCN 2)mRNA和蛋白表达的影响,探讨电针经穴效应及后效应的相对特异性.方法:SD大鼠随机分为正常组、模型组、电针手少阴心经神门组(简称神门组)、电针手太阴肺经太渊组(简称太渊组),每组12只.采用冠状动脉前降支结扎法复制AMI模型.两电针组分别电针"神门""太渊"穴,刺激15 min,1次/d,共治疗7d.于末次电针治疗后即刻和次日相同时间各取材6只大鼠,分别称为神门8d组、神门9d组、太渊8 d组和太渊9 d组.采用实时荧光定量PCR法检测心肌组织HCN 2 mRNA表达水平,Western blot法检测HCN 2蛋白表达水平.结果:与正常组比较,模型组HCN 2 mRNA和蛋白表达水平均显著减少(P<0.01);与模型组比较,神门组及太渊组HCN 2 mRNA和蛋白表达量均显著增多(P<0.01);与神门8 d组比较,神门9 d组的HCN 2 mRNA和蛋白表达量均显著增多(P<0.01),但太渊8 d组的HCN 2 mR-NA和蛋白表达量均减少(P<0.01);与神门9 d组比较,太渊9 d组的HCN 2 mRNA和蛋白表达量均显著减少(P<0.01);与太渊8 d组比较,太渊9 d组HCN 2蛋白表达显著增加(P<0.01).结论:电针心经和肺经均可以促进AMI模型大鼠心肌组织HCN 2 mRNA和蛋白的表达,且具有一定的持续性后效应,电针心经在调整心肌组织HCN 2表达中具有相对特异性.%Objective To observe the effect of electroacupuncture (EA) stimulation of Heart and Lung meridians on the expression of hyperpolarization-activated cyclic nucleotide gated potassium channel 2 (HCN 2) mRNA and protein in the myocar-dium of the left ventricle in acute myocardial ischemia (AMI) rats ,so as to underlying its mechanism in improving ischemic myo-cardial injury .Methods A total of 120 male SD rats were randomly divided into normal control ,model ,EA-Heart Meridian (HT)andEA-LungMeridian(LU)groups(n=30ratsineachgroup).TheAMImodelwasestablishedbyocclusionoftheanterior descending branch of coronary artery .EA (1 mA ,10 Hz) was applied to bilateral "Shenmen"(HT 7) or"Taiyuan"(LU 9) and the spot about 1 mm apart from the HT 7 or LU 9 on the axopetal end for 15 min on the second day after modeling ,once a day for 7 days .The left ventricular myocardium tissue was sampled for determining the expression levels of HCN 2 mRNA and protein with qPCR and Western blot ,separately .Results After AMI ,both HCN 2 mRNA and protein expression levels in the model group were significantly decreased in comparison with the normal control group ( P<0 .01) .Following EA intervention ,the expression levels of HCN 2 mRNA and protein expressions were significantly up-regulated relevant to the model group ( P<0 .01) .The effects were more obvious in the EA-HT group than in the EA-LU group ,and the expression levels were higher in the left ventricular myocardium tissue sampled on the next day after the treatment than those in the tissue sampled immediately after the treatment ( P<0 .01) .Conclusion Both EA stimulation of Heart Meridian and Lung Meridian can improve the expression of HCN 2 mRNA and protein in the myocardium in AMI rats ,which existing a delayed effect and meridian specificity .
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