Effect of high salinity on cell growth and protein production of Antarctic ice microalgae Chlamydomonas sp. ICE-L

郑洲; 缪锦来; 阚光峰; 金青; 丁燏; 刘芳明; 王守强; 王以斌

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Effect of high salinity on cell growth and protein production of Antarctic ice microalgae Chlamydomonas sp. ICE-L

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Antarctic ice microalgae Chlamydomonas sp.ICE-L can survive and thrive in Antarctic sea ice.In this study,Chlamydomonas sp.ICE-L could survive at the salinity of 132‰ NaCl.SDS-PAGE showed that the density of 2 bands(26 and 36 kD) decreased obviously at the salinity of 99‰ NaCl compared to at the salinity of 33‰ NaCl.The soluble proteins in Chlamydomonas sp.ICE-L grown under salinity of 33‰ and 99% NaCl were compared by 2-D gel electrophoresis.After shocking with high salinity,8 protein spots were found to disappear,and the density of 28 protein spots decreased.In addition,19 protein spots were enhanced or induced,including one new peptide(51 kD).The changes of proteins might be correlated with the resistance for Chlamydomonas sp.ICE-L to high salinity.

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《极地科学进展：英文版》 |2010年第1期|81-90|共10页
作者
郑洲; 缪锦来; 阚光峰; 金青; 丁燏; 刘芳明; 王守强; 王以斌;
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作者单位

Key Laboratory of Marine Bio-active Substances;

SOA;

College of Oceanology;

Weihai Branch of Harbin University of Technology;

College of Chemical Engineering;

Qingdao University of Science & Technology;

Ocean University of Guangdong;

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原文格式 PDF
正文语种 chi
中图分类藻类;植物细胞的离体培养;
关键词
南极冰藻; 细胞生长; 蛋白质; 含盐量; 衣藻; 生产; 可溶性蛋白; 高盐度;

机译：南极冰藻;细胞生长;蛋白质;含盐量;衣藻;生产;可溶性蛋白;高盐度;

Effect of high salinity on cell growth and protein production of Antarctic ice microalgae Chlamydomonas sp. ICE-L

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