[Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [Method]Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials to analyze the effects of media type,cytokinin type and concentration,carbon source type and concentration,and light intensity on tissue culture and propagation of Benihoppe strawberry. [Result] MS was the optimal media for plantlet propagation. In the media containing 1. 2 mg / L BA( with addition of 0. 1 mg / L NAA),fresh weight,dry weight and propagation coefficient of strawberry plantlets reached the maximum,which were 2. 259 g,0. 221 g and 12. 4,respectively. The optimal carbon source was 30 g / L sucrose,and the optimal light intensity was 1 600 lx. [Conclusion] The best media for tissue culture and rapid propagation of Benihoppe strawberry was MS + BA 1. 2 mg / L + NAA 0. 1 mg / L + sucrose 30 g / L + agar 8g / L. This study provided scientific basis for large-scale production of Benihoppe strawberry.
展开▼
