首页> 中文期刊> 《农业生物技术:英文版》 >Effects of Acetosyringone on Genetic Transformation of Waxy Maize Using Different Receptor Systems

Effects of Acetosyringone on Genetic Transformation of Waxy Maize Using Different Receptor Systems

         

摘要

Acetosyringone( AS) has great influences on Agrobacterium-mediated genetic transformation. In order to improve the transformation frequency of waxy maize,in this study,shoot tips and immature embryo-derived callus of Lainongnuo 38 were collected as receptor materials,to compare the effects of pH in suspension medium,addition modes and concentrations of acetosyringone on genetic transformation of waxy maize. Results showed that adding 2 μl of 150 μmol / L AS in the wound with suspension medium at pH 5. 2 can significantly improve the transformation frequency of shoot tips of waxy maize; in genetic transformation of maize callus,adding 5 mg / L AS in infection solution can significantly improve the percentage of resistant callus.

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