Study on Surface Sterilization Method for Tissue Culture of Haloxylon ammodendron （ C.A. Mey. ） Bunge Seeds

Dong WANG; Guiping ZHAO

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Study on Surface Sterilization Method for Tissue Culture of Haloxylon ammodendron （ C.A. Mey. ） Bunge Seeds

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[Objective] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron(C.A.Mey.).[Method] H.ammodendron(C.A.Mey.) seeds were used as experimental materials to investigate the sterilization results of different sterilization treatments and the effect of different seed sizes on the survival rate of aseptic seedlings.[Result] Sterilization of H.ammodendron(C.A.Mey.) seeds achieved the best result by using 75% alcohol for 15 min and 0.1% mercuric chloride for 8 min.Seeds with diameter >2.0 mm were used as explants and achieved relatively high survival rate of aseptic seedlings,which reached above 65%.[Conclusion] This study established a surface sterilization method for tissue culture of H.ammodendron(C.A.Mey.) Bunge seeds.

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《农业生物技术：英文版》 |2012年第003期|P.26-28|共3页
作者
Dong WANG; Guiping ZHAO;
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作者单位

^pLaboratory;

of;

Tarim;

Animal;

Husbandry;

Science;

and;

Technology,;

Xinjiang;

Production;

＆;

Construction;

Group/College;

of;

Animal;

Science,;

Tarim;

University,;

Alar;

843300,;

China;

^pSheep;

Breeding;

Field,;

Baicheng;

842300,;

China;

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原文格式 PDF
正文语种 CHI
中图分类大豆;
关键词
种子大小; 灭菌方法; 组织培养; 梭梭; 表面; CA; 实验材料; 灭菌处理;

机译：种子大小;灭菌方法;组织培养;梭梭;表面;CA;实验材料;灭菌处理;

Study on Surface Sterilization Method for Tissue Culture of Haloxylon ammodendron （ C.A. Mey. ） Bunge Seeds

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