Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t

Jing SU; Wenjuan WANG; Shen CHEN; Congying WANG; Jianyuan YANG; Aiqing Feng; Xiaoyuan ZHU

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Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t

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Specific molecular markers for detecting the alleles of Pi2/Pi9/Piz-t,designated as Pi2 SNP,Pi9 SNP and Pizt-PA,respectively,have been successfully developed through sequence comparison method.These three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus,which facilitates the molecular marker-assistant selection and the development of pyramiding cultivars combined with other allele R genes.Additionally,101 main cultivars and breeding parents collected from different rice-planting areas have been diagnosed with these three specific markers,and the results showed that only 2 out of the 101 cultivars carried Piz-t,but neither Pi2 nor Pi9 band had been detected.It indicates that most of the main cultivars and breeding parents in China do not carry Pi2/Pi9/Piz-t.These results provide important information for the application of Pi2/Pi9/Piz-t in the blast resistance breeding programs.

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《农业生物技术：英文版》 |2020年第5期|P.9-1217|共5页
作者
Jing SU; Wenjuan WANG; Shen CHEN; Congying WANG; Jianyuan YANG; Aiqing Feng; Xiaoyuan ZHU;
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作者单位

The Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 ChinaGuangdong Provincial Key Laboratory of High Technology for Plant Protection Guangzhou 510640 China;

The Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 ChinaGuangdong Provincial Key Laboratory of High Technology for Plant Protection Guangzhou 510640 China;

The Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 ChinaGuangdong Provincial Key Laboratory of High Technology for Plant Protection Guangzhou 510640 China;

The Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 ChinaGuangdong Provincial Key Laboratory of High Technology for Plant Protection Guangzhou 510640 China;

The Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 China;

The Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 China;

The Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 China;

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原文格式 PDF
正文语种 chi
中图分类禾谷类作物;
关键词
Rice; Blast; Resistance gene; Molecular marker; MAS; Pi2/9 locus;

机译：米饭;爆炸;抗性基因;分子标记;MAS;PI2 / 9基因座;

Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t

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