Prokaryotic Expression and Preparation of Polyclonal Antibody of HtrA Protein from Escherichia coli Causing Pneumonia in Fox

Zhang Zhiqiang; Wu Tonglei; Wu Qingmin; Yan Xijun; Bai Xue; Zhang Yanling; He Hongxuan; Zhang Hongxue; Liu Changhao; Shi Qiumei

首页> 中文期刊> 《动物与饲料科学：英文版》 >Prokaryotic Expression and Preparation of Polyclonal Antibody of HtrA Protein from Escherichia coli Causing Pneumonia in Fox

Prokaryotic Expression and Preparation of Polyclonal Antibody of HtrA Protein from Escherichia coli Causing Pneumonia in Fox

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To investigate the roles of HtrA protein in bacterial adaption to stresses, htra gene was cloned from an Escherichia coli strain isolated from silver fox, double digested, and ligated into the prokaryotic expression vector pET32a. Then, the recombinant plasmid pET32a-htra was transformed into E. coli BL21 competent cells. The over-expression of His-HtrA protein was induced by the addition of IPTG at a final concentration of 1 mmol/L, and verified by SDA-PAGE and Western blot. In addition, the protein was purified with Ni-NTA agarose, and used to immunize mice,and the polyclonal antibody specifically bound to E. coli HtrA protein. The results will provide a theoretical basis for studying the roles of HtrA protein and developing vaccines for fur-bearing animals against E. coli.

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《动物与饲料科学：英文版》 |2018年第001期|P.58-61|共4页
作者
Zhang Zhiqiang; Wu Tonglei; Wu Qingmin; Yan Xijun; Bai Xue; Zhang Yanling; He Hongxuan; Zhang Hongxue; Liu Changhao; Shi Qiumei;
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作者单位

[1]Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology,Changli 066600;

[1]Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology,Changli 066600;

[2]China Agricultural University,Beijing 100193;

[3]Key Laboratory of Economic Animal Diseases of Ministry of Agriculture, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences,Changchun 130112,China;

[3]Key Laboratory of Economic Animal Diseases of Ministry of Agriculture, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences,Changchun 130112,China;

[3]Key Laboratory of Economic Animal Diseases of Ministry of Agriculture, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences,Changchun 130112,China;

[4]Institute of Zoology, Chinese Academy of Sciences,Beijing 100101,China;

[5]Qilu Animal Health Products Co., Ltd.,Jinan 250100,China;

[5]Qilu Animal Health Products Co., Ltd.,Jinan 250100,China;

[1]Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology,Changli 066600;

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原文格式 PDF
正文语种 CHI
中图分类微生物遗传学;
关键词
原核生物; 蛋白质; 抗体; 肺病; IPTG; SDA; 细菌; 孤立;

机译：原核生物;蛋白质;抗体;肺病;IPTG;SDA;细菌;孤立;

Prokaryotic Expression and Preparation of Polyclonal Antibody of HtrA Protein from Escherichia coli Causing Pneumonia in Fox

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