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首页> 中文期刊> 《动物与饲料科学：英文版》 >Observation on Culture Characteristics of Mycoplasma bovis with Four Different Antigen Quantification Methods

Observation on Culture Characteristics of Mycoplasma bovis with Four Different Antigen Quantification Methods

         
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[Objective] The paper was to explore the antigen harvest time of Mycoplasma bovis and the antigen quantification alternative method. [Method]M. bovis 08 M strain was inoculated in the Thiaucourt's medium containing 10% horse serum. Four growth curves were plotted by simultaneously measuring color change units( CCU),colony forming units( CFU),protein concentration and nucleic acid levels within 110 h. [Result]The growth of M. bovis was divided into four phases: the longarithmic phase appeared after being cultured for 10 h; the stationary phase appeared at 30 h with the highest number of viable cells up to1.0 × 108CCU/mL and 7.7 × 107CCU/mL,respectively; and the decline phase started at 75 h. The protein concentration of M. bovis increased rapidly from 15 to35 h,reached 72.06 μg/mL at 35 h,then maintained at 53.38-70. 65 μg/mL. The nucleic acid levels of M. bovis increased rapidly from 15 h,and the cycle threshold( Ct) values were maintained between 15.32 and 17.84 after 25 h. [Conclusion]There was a good correlation between the protein concentration and variable count of M. bovis at the early stationary phase,which was the best time period to harvest antigen. The protein concentration determination could be an alternative method to quantify antigen content of M. bovis when preparing inactivated M. bovis vaccine.

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